bioruptor plustm (DIAGENODE DIAGNOSTICS)
90
Structured Review
DIAGENODE DIAGNOSTICS
bioruptor plustm
Bioruptor Plustm, supplied by DIAGENODE DIAGNOSTICS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bioruptor+plustm/bioruptor+pico/10__1016_slash_j__scienta__2024__113865-104-34-36
Average 90 stars, based on 1 article reviews
Bioruptor Plustm, supplied by DIAGENODE DIAGNOSTICS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bioruptor+plustm/bioruptor+pico/10__1016_slash_j__scienta__2024__113865-104-34-36
Average 90 stars, based on 1 article reviews
bioruptor plustm - by Bioz Stars,
2026-10
90/100 stars
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Saline:Article Title: Identifying Cell-Type-Specific Metabolic Signatures Using Transcriptome and Proteome Analyses. Article Snippet: .. Materials Intestinal crypts from step 17 of Basic Protocol 1 Crypt digestion buffer (see recipe; prepare 3 ml/sample) Phosphate-buffered saline (PBS; PBS Tablets; Gibco, cat. no. 18912014, to be dissolved in 500 ml deionized water; keep on ice) Y-27632 dihydrochloride monohydrate (Abcam ab120129) 2× proteomics lysis buffer (see recipe) 1 M dithiothreitol (DTT; Roth 6908.3) 200 mM iodoacetamide (Sigma I1149); prepare fresh directly before use and keep in the dark (cannot be stored)Gebert et al. 8 of 29 Current Protocols Acetone (Biosolve 0001037801BS), ice-cold HPLC water (Sigma 270733) Protein digestion buffer (see recipe) LysC stock solution (see recipe) Trypsin stock solution (see recipe) 10% trifluoroacetic acid (Biosolve 0020234131BS) Acetonitrile (Biosolve 0001204102BS) Formic acid (Roth 4724.3) HRM kit peptides (Biognosys AG 42896) 50-ml conical tubes (e.g., Corning Falcon) 1.5-ml microcentrifuge tubes (Eppendorf 0030120.086) 37°C water bath Centrifuge with temperature control (Eppendorf 5804R) 70-μm Lysis:Article Title: Identifying Cell-Type-Specific Metabolic Signatures Using Transcriptome and Proteome Analyses. Article Snippet: .. Materials Intestinal crypts from step 17 of Basic Protocol 1 Crypt digestion buffer (see recipe; prepare 3 ml/sample) Phosphate-buffered saline (PBS; PBS Tablets; Gibco, cat. no. 18912014, to be dissolved in 500 ml deionized water; keep on ice) Y-27632 dihydrochloride monohydrate (Abcam ab120129) 2× proteomics lysis buffer (see recipe) 1 M dithiothreitol (DTT; Roth 6908.3) 200 mM iodoacetamide (Sigma I1149); prepare fresh directly before use and keep in the dark (cannot be stored)Gebert et al. 8 of 29 Current Protocols Acetone (Biosolve 0001037801BS), ice-cold HPLC water (Sigma 270733) Protein digestion buffer (see recipe) LysC stock solution (see recipe) Trypsin stock solution (see recipe) 10% trifluoroacetic acid (Biosolve 0020234131BS) Acetonitrile (Biosolve 0001204102BS) Formic acid (Roth 4724.3) HRM kit peptides (Biognosys AG 42896) 50-ml conical tubes (e.g., Corning Falcon) 1.5-ml microcentrifuge tubes (Eppendorf 0030120.086) 37°C water bath Centrifuge with temperature control (Eppendorf 5804R) 70-μm Article Title: Transcription factor Constans-like 4 may regulate tomato fruit ripening by interacting with abscisic acid and ethylene signaling Article Snippet: .. The sediment obtained was mixed with 0.4 mL of nucleic acid lysis buffer containing protease inhibitors, lysed on ice for 30 min, and sonicated into fragments ranging from 200 to 700 bp with a High Performance Liquid Chromatography:Article Title: Identifying Cell-Type-Specific Metabolic Signatures Using Transcriptome and Proteome Analyses. Article Snippet: .. Materials Intestinal crypts from step 17 of Basic Protocol 1 Crypt digestion buffer (see recipe; prepare 3 ml/sample) Phosphate-buffered saline (PBS; PBS Tablets; Gibco, cat. no. 18912014, to be dissolved in 500 ml deionized water; keep on ice) Y-27632 dihydrochloride monohydrate (Abcam ab120129) 2× proteomics lysis buffer (see recipe) 1 M dithiothreitol (DTT; Roth 6908.3) 200 mM iodoacetamide (Sigma I1149); prepare fresh directly before use and keep in the dark (cannot be stored)Gebert et al. 8 of 29 Current Protocols Acetone (Biosolve 0001037801BS), ice-cold HPLC water (Sigma 270733) Protein digestion buffer (see recipe) LysC stock solution (see recipe) Trypsin stock solution (see recipe) 10% trifluoroacetic acid (Biosolve 0020234131BS) Acetonitrile (Biosolve 0001204102BS) Formic acid (Roth 4724.3) HRM kit peptides (Biognosys AG 42896) 50-ml conical tubes (e.g., Corning Falcon) 1.5-ml microcentrifuge tubes (Eppendorf 0030120.086) 37°C water bath Centrifuge with temperature control (Eppendorf 5804R) 70-μm Control:Article Title: Identifying Cell-Type-Specific Metabolic Signatures Using Transcriptome and Proteome Analyses. Article Snippet: .. Materials Intestinal crypts from step 17 of Basic Protocol 1 Crypt digestion buffer (see recipe; prepare 3 ml/sample) Phosphate-buffered saline (PBS; PBS Tablets; Gibco, cat. no. 18912014, to be dissolved in 500 ml deionized water; keep on ice) Y-27632 dihydrochloride monohydrate (Abcam ab120129) 2× proteomics lysis buffer (see recipe) 1 M dithiothreitol (DTT; Roth 6908.3) 200 mM iodoacetamide (Sigma I1149); prepare fresh directly before use and keep in the dark (cannot be stored)Gebert et al. 8 of 29 Current Protocols Acetone (Biosolve 0001037801BS), ice-cold HPLC water (Sigma 270733) Protein digestion buffer (see recipe) LysC stock solution (see recipe) Trypsin stock solution (see recipe) 10% trifluoroacetic acid (Biosolve 0020234131BS) Acetonitrile (Biosolve 0001204102BS) Formic acid (Roth 4724.3) HRM kit peptides (Biognosys AG 42896) 50-ml conical tubes (e.g., Corning Falcon) 1.5-ml microcentrifuge tubes (Eppendorf 0030120.086) 37°C water bath Centrifuge with temperature control (Eppendorf 5804R) 70-μm Extraction:Article Title: Identifying Cell-Type-Specific Metabolic Signatures Using Transcriptome and Proteome Analyses. Article Snippet: .. Materials Intestinal crypts from step 17 of Basic Protocol 1 Crypt digestion buffer (see recipe; prepare 3 ml/sample) Phosphate-buffered saline (PBS; PBS Tablets; Gibco, cat. no. 18912014, to be dissolved in 500 ml deionized water; keep on ice) Y-27632 dihydrochloride monohydrate (Abcam ab120129) 2× proteomics lysis buffer (see recipe) 1 M dithiothreitol (DTT; Roth 6908.3) 200 mM iodoacetamide (Sigma I1149); prepare fresh directly before use and keep in the dark (cannot be stored)Gebert et al. 8 of 29 Current Protocols Acetone (Biosolve 0001037801BS), ice-cold HPLC water (Sigma 270733) Protein digestion buffer (see recipe) LysC stock solution (see recipe) Trypsin stock solution (see recipe) 10% trifluoroacetic acid (Biosolve 0020234131BS) Acetonitrile (Biosolve 0001204102BS) Formic acid (Roth 4724.3) HRM kit peptides (Biognosys AG 42896) 50-ml conical tubes (e.g., Corning Falcon) 1.5-ml microcentrifuge tubes (Eppendorf 0030120.086) 37°C water bath Centrifuge with temperature control (Eppendorf 5804R) 70-μm Polymerase Chain Reaction:Article Title: Identifying Cell-Type-Specific Metabolic Signatures Using Transcriptome and Proteome Analyses. Article Snippet: .. Materials Intestinal crypts from step 17 of Basic Protocol 1 Crypt digestion buffer (see recipe; prepare 3 ml/sample) Phosphate-buffered saline (PBS; PBS Tablets; Gibco, cat. no. 18912014, to be dissolved in 500 ml deionized water; keep on ice) Y-27632 dihydrochloride monohydrate (Abcam ab120129) 2× proteomics lysis buffer (see recipe) 1 M dithiothreitol (DTT; Roth 6908.3) 200 mM iodoacetamide (Sigma I1149); prepare fresh directly before use and keep in the dark (cannot be stored)Gebert et al. 8 of 29 Current Protocols Acetone (Biosolve 0001037801BS), ice-cold HPLC water (Sigma 270733) Protein digestion buffer (see recipe) LysC stock solution (see recipe) Trypsin stock solution (see recipe) 10% trifluoroacetic acid (Biosolve 0020234131BS) Acetonitrile (Biosolve 0001204102BS) Formic acid (Roth 4724.3) HRM kit peptides (Biognosys AG 42896) 50-ml conical tubes (e.g., Corning Falcon) 1.5-ml microcentrifuge tubes (Eppendorf 0030120.086) 37°C water bath Centrifuge with temperature control (Eppendorf 5804R) 70-μm Mass Spectrometry:Article Title: Identifying Cell-Type-Specific Metabolic Signatures Using Transcriptome and Proteome Analyses. Article Snippet: .. Materials Intestinal crypts from step 17 of Basic Protocol 1 Crypt digestion buffer (see recipe; prepare 3 ml/sample) Phosphate-buffered saline (PBS; PBS Tablets; Gibco, cat. no. 18912014, to be dissolved in 500 ml deionized water; keep on ice) Y-27632 dihydrochloride monohydrate (Abcam ab120129) 2× proteomics lysis buffer (see recipe) 1 M dithiothreitol (DTT; Roth 6908.3) 200 mM iodoacetamide (Sigma I1149); prepare fresh directly before use and keep in the dark (cannot be stored)Gebert et al. 8 of 29 Current Protocols Acetone (Biosolve 0001037801BS), ice-cold HPLC water (Sigma 270733) Protein digestion buffer (see recipe) LysC stock solution (see recipe) Trypsin stock solution (see recipe) 10% trifluoroacetic acid (Biosolve 0020234131BS) Acetonitrile (Biosolve 0001204102BS) Formic acid (Roth 4724.3) HRM kit peptides (Biognosys AG 42896) 50-ml conical tubes (e.g., Corning Falcon) 1.5-ml microcentrifuge tubes (Eppendorf 0030120.086) 37°C water bath Centrifuge with temperature control (Eppendorf 5804R) 70-μm Sonication:Article Title: Genome-wide (ChIP-seq) identification of target genes regulated by BdbZIP10 during paraquat-induced oxidative stress Article Snippet: Chromatin was prepared using the Zymo-SpinTM ChIP kit (Zymo Research Corp., Irvine, CA) following their modified protocol for isolation of plant chromatin. .. Sonication was performed at high power setting for 40 cycles (30 s on, 30 s off) using a Article Title: Transcription factor Constans-like 4 may regulate tomato fruit ripening by interacting with abscisic acid and ethylene signaling Article Snippet: .. The sediment obtained was mixed with 0.4 mL of nucleic acid lysis buffer containing protease inhibitors, lysed on ice for 30 min, and sonicated into fragments ranging from 200 to 700 bp with a Article Title: Genome-wide (ChIP-seq) identification of target genes regulated by BdbZIP10 during paraquat-induced oxidative stress. Article Snippet: Chromatin was prepared using the Zymo-SpinTM ChIP kit (Zymo Research Corp., Irvine, CA) following their modified protocol for isolation of plant chromatin. .. Sonication was performed at high power setting for 40 cycles (30 s on, 30 s off ) using a |